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Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS)

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dc.contributor.author Bellato, Ana Cláudia S.
dc.contributor.author Menegário, Amauri A.
dc.contributor.author Giné, Maria Fernanda
dc.date.accessioned 2022-04-02T13:43:32Z
dc.date.available 2022-04-02T13:43:32Z
dc.date.issued 2003
dc.identifier.citation BELLATO, A. C. S.; MENEGÁRIO, A. A.; GINÉ, M. F. Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS). Journal of the Brazilian Chemical Society, São Paulo, v. 14, n. 2, p. 263-273, mar./abr. 2003. pt_BR
dc.identifier.issn 1678-4790
dc.identifier.uri https://doi.org/10.1590/S0103-50532003000200014 pt_BR
dc.identifier.uri http://www.sbicafe.ufv.br/handle/123456789/13413
dc.description.abstract Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 µL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 µg g-1. The detection limit (3σ) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively. pt_BR
dc.format pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Sociedade Brasileira de Química pt_BR
dc.relation.ispartofseries Journal of the Brazilian Chemical Society;v.14, n.2, 2003
dc.rights Open Access pt_BR
dc.subject Isotope dilution pt_BR
dc.subject Inductively coupled plasma mass spectrometry pt_BR
dc.subject Biological cell fractionation pt_BR
dc.subject Boron isotope ratios pt_BR
dc.subject Plant analysis pt_BR
dc.subject.classification Cafeicultura::Solos e nutrição do cafeeiro pt_BR
dc.title Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS) pt_BR
dc.type Artigo pt_BR

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