dc.contributor.author |
Bellato, Ana Cláudia S. |
|
dc.contributor.author |
Menegário, Amauri A. |
|
dc.contributor.author |
Giné, Maria Fernanda |
|
dc.date.accessioned |
2022-04-02T13:43:32Z |
|
dc.date.available |
2022-04-02T13:43:32Z |
|
dc.date.issued |
2003 |
|
dc.identifier.citation |
BELLATO, A. C. S.; MENEGÁRIO, A. A.; GINÉ, M. F. Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS). Journal of the Brazilian Chemical Society, São Paulo, v. 14, n. 2, p. 263-273, mar./abr. 2003. |
pt_BR |
dc.identifier.issn |
1678-4790 |
|
dc.identifier.uri |
https://doi.org/10.1590/S0103-50532003000200014 |
pt_BR |
dc.identifier.uri |
http://www.sbicafe.ufv.br/handle/123456789/13413 |
|
dc.description.abstract |
Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 µL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 µg g-1. The detection limit (3σ) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively. |
pt_BR |
dc.format |
pdf |
pt_BR |
dc.language.iso |
en |
pt_BR |
dc.publisher |
Sociedade Brasileira de Química |
pt_BR |
dc.relation.ispartofseries |
Journal of the Brazilian Chemical Society;v.14, n.2, 2003 |
|
dc.rights |
Open Access |
pt_BR |
dc.subject |
Isotope dilution |
pt_BR |
dc.subject |
Inductively coupled plasma mass spectrometry |
pt_BR |
dc.subject |
Biological cell fractionation |
pt_BR |
dc.subject |
Boron isotope ratios |
pt_BR |
dc.subject |
Plant analysis |
pt_BR |
dc.subject.classification |
Cafeicultura::Solos e nutrição do cafeeiro |
pt_BR |
dc.title |
Boron isotope dilution in cellular ractions of coffee leaves evaluated by inductively coupled plasma mass spectrometry with direct injection nebulization (DIN-ICP-MS) |
pt_BR |
dc.type |
Artigo |
pt_BR |